Glyco-Skills development core

Workshop: Sialoglycan isolation and recogntion &
Siglec recogntion of lung mucins

Johns Hopkins University School of Medicine – June 9-11, 2014

Workshop Objectives & Syllabus

Mucin structure, distribution & function (Ronald Schnaar) Lecture

Brain ganglioside structures & functions (Ronald Schnaar) Lecture

Module 1: Agarose-acrylamide composite gels // Human lung mucins // Mouse brain gangliosides

Module 2: Quantitative mucin dot-blot

Module 3: TLC of brain gangliosides // Glycolipid ELISA

Module 4: Lung lectin-overlay histochemistry