Glyco-Skills development core

Trachea Isolation, Lung Epithelium Cell Culture
and Lung Tissue Immunohistochemistry Workshop

The Johns Hopkins Asthma and Allergy Center – July 24-26, 2012

Workshop Syllabus

Mouse Airways (Zhou Zhu) Lecture & Video

Human Airways (Bruce Bochner) Lecture & Video

Epithelial Cell Air-liquid Interface Culture (Becky Vonakis) Lecture & Video

Lab Safety & Tissue Banking (Allen Myers) Lecture & Video

Protocol 1: Mouse Tracheal Epithelial Cell Culture

Protocol 2: Sterile Technique

Protocol 3: Human Tracheal Epithelial Cell Culture

Protocol 4: Lung Tissue Immunohistochemistry

Protocol 5: Lung Exudate

Video: Epithelial Cell Smoke Chamber